Search results for "Molecular method"

showing 7 items of 7 documents

Cocirculation of Hajj and non-Hajj strains among serogroup W meningococci in Italy, 2000 to 2016

2019

In Italy, B and C are the predominant serogroups among meningococci causing invasive diseases. Nevertheless, in the period from 2013 to 2016, an increase in serogroup W Neisseria meningitidis (MenW) was observed. This study intends to define the main characteristics of 63 MenW isolates responsible of invasive meningococcal disease (IMD) in Italy from 2000 to 2016. We performed whole genome sequencing on bacterial isolates or single gene sequencing on culture-negative samples to evaluate molecular heterogeneity. Our main finding was the cocirculation of the Hajj and the South American sublineages belonging to MenW/clonal complex (cc)11, which gradually surpassed the MenW/cc22 in Italy. All M…

0301 basic medicineSerotypeMaleCefotaximeinvasive bacterial infectionsEpidemiologymolecular methodsNeisseria meningitidismedicine.disease_causeDisease Outbreaks0302 clinical medicineGenotypemolecular method030212 general & internal medicinenational surveillance systemChildPhylogenyAged 80 and overSurveillanceNeisseria meningitidisitaly; neisseria meningitidis; capsular serogroup w; clonal complex 11; invasive bacterial infections; invasive meningococcal disease; molecular methods; national surveillance systeminvasive bacterial infectionMiddle Aged3. Good healthItalyChild PreschoolPopulation SurveillanceFemalePublic Healthmedicine.drugAdultAdolescentAntibiotic sensitivity030106 microbiologyBiologySerogroup03 medical and health sciencesYoung AdultNeisseria meningitidis Serogroup W-135VirologymedicineNeisseria meningitidiHumanscapsular serogroup WAgedWhole Genome Sequencinginvasive meningococcal diseaseEnvironmental and Occupational HealthPublic Health Environmental and Occupational HealthInfant NewbornInfantSequence Analysis DNAVirologyPenicillinMeningococcal Infectionsclonal complex 11capsular serogroup W; clonal complex 11; invasive bacterial infections; invasive meningococcal disease; Italy; molecular methods; national surveillance system; Neisseria meningitidis; Epidemiology; Public Health Environmental and Occupational Health; VirologyHajjRifampicin
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Virus entéricos humanos en alimentos: detección y métodos de inactivación

2020

[ES] Los principales patógenos víricos que podemos adquirir ingiriendo alimentos contaminados son los norovirus, el virus de la hepatitis A y el virus de la hepatitis E que se propagan principalmente a través de la vía fecal oral. En los últimos años, la incidencia de brotes de transmisión alimentaria causados por estos patógenos ha experimentado un aumento considerable, en parte debido al comercio globalizado y a los cambios en los hábitos de consumo. Las matrices alimentarias que mayor riesgo representan para el consumidor son los moluscos bivalvos, vegetales de IV gama, frutas tipo baya y platos listos para comer. Actualmente las técnicas moleculares son las más habituales para la detecc…

Cultural StudiesSociology and Political ScienceFood industryViral inactivationmolecular methodsFoodborne virusesBiologymedicine.disease_causeGeneral WorksFood safety03 medical and health sciencesseguridad alimentariaAmedicineenvases virucidas030304 developmental biologyinactivación víricaInfectivityviral inactivationmetagenomicscompuestos virucidas0303 health sciencesantiviral packaging030306 microbiologybusiness.industryvirus entéricosGeneral Arts and Humanitiesdigestive oral and skin physiologyfoodborne virusesFoodborne outbreakHepatitis AFood safetymedicine.diseaseVirologyantiviral compoundsVirusMolecular methodsfood safetymétodos molecularesAliments ContaminacióViral genomesAntiviral packagingmetagenómicaNorovirusAntiviral compoundsMetagenomicsbusinessContaminated foodArbor
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The use of multiplex PCR to detect and differentiate food- and beverage-associated microorganisms: a review.

2007

Regarding food safety, rapid detection of microbial species is crucial to develop effective preventive and/or adjustment measures. Classical methods for determining the presence of certain species are time-consuming and labor-intensive, hence, molecular methods, which offer speed, sensitivity and specificity, have been developed to address this problem. Multiplex PCR (MPCR) is widely applied in the various fields of microbiology for the rapid differentiation of microbial species without compromising accuracy. This paper describes the method and reports on the state-of-the-art application of this technique to the identification of microorganisms vehiculated with foods and beverages. The iden…

Microbiology (medical)MicroorganismBiologyMicrobiologyRapid detectionPolymerase Chain ReactionBeveragesMicrobial differentiationSimultaneous detectionMultiplex polymerase chain reactionFood microbiologyBeverageMolecular BiologyRapid identificationEcosystemBacteriabusiness.industryProbioticsWaterMolecular methodMultiplex PCRFood safetyBiotechnologyRapid identificationFoodFood MicrobiologyIdentification (biology)businessSettore AGR/16 - Microbiologia AgrariaJournal of microbiological methods
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Molecular analyses of soil denitrifying bacteria

2006

[SDV] Life Sciences [q-bio][SDE] Environmental Sciences[SDV]Life Sciences [q-bio][SDE]Environmental SciencesFUNCTIONAL GROUP LEVELSPLANT MICROORGANISMSMOLECULAR METHODS
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Geny warunkujące produkcję mykotoksyn u grzybów strzępkowych obecnych w magazynach zbożowych

2016

mycotoxinsfilamentous fungimykotoksynymolecular methodsgrzyby strzępkowemetody molekularne
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Root and crown rot of olive caused by Phytophthora spp

2011

phylogeneticsRoot and crown rot olive Phytophthora sppfoot rotSettore AGR/12 - Patologia Vegetalefoot rot ; emerging diseases; molecular methods; phylogeneticsemerging diseasesmolecular methods
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Molecular methods to evaluate AMF functionality in soil and in roots

2005

International audience

roots[SDV] Life Sciences [q-bio][SDV]Life Sciences [q-bio]Molecular methods to evaluate AMF functionalityComputingMilieux_MISCELLANEOUSsoil
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